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Reporter genes are attached to a regulatory sequence of a gene of interest. When expressed by the host organism, they generate measurable, easily identifiable characteristics that can be used as an indication of whether a gene has been taken up or expressed.
The Proteasome-Glo™ Cell-Based Assays are homogeneous, luminescent assays that measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome complex in cultured cells.
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The Kinase-Glo™ Luminescent Kinase Assays are homogeneous non-radioactive systems for determining the activity of purified kinases by quantifying the amount of ATP remaining in solution following a kinase reaction.
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Recombinant human EGFR (amino acids 695-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity.
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The Kinase-Glo™ Luminescent Kinase Assays are homogeneous non-radioactive systems for determining the activity of purified kinases by quantifying the amount of ATP remaining in solution following a kinase reaction.
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More
UDP-sugar substrates are used with the UDP-Glo Glycosyltransferase Assay to detect the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
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The UDP-Glo Glycosyltransferase Assay is a bioluminescent assay for detecting the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
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The UDP-Glo Glycosyltransferase Assay is a bioluminescent assay for detecting the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
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The mFcgammaRIV ADCC Reporter Bioassay is a biologically relevant MOA-based assay that can be used to measure the activity of mouse antibodies that specifically bind and activate FcgammaRIV.
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The GTPase-Glo Assay assesses the activities of GTPases, GAPs and GEFs, which are components of the GTPase cycle, by detecting the amount of GTP remaining after GTP hydrolysis in a GTPase reaction.
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The ADP-Glo™ Max Assay is a luminescent ADP detection assay that provides a universal, homogeneous, high-throughput screening method to measure ATPase or kinase activity by quantifying the amount of ADP produced in a reaction.
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Succinate-Glo JmjC Demethylase/Hydroxylase Assay is a bioluminescent assay for detecting the activity of JumonjiC (JmjC) histone demethylases hydroxylases.
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PCR Optimization Kit is a portfolio of preformulated high-quality buffers, 25mM MgCl2 and GoTaq MDx Hot Start Polymerase. Perform a series of short experiments to quickly survey which combination will yield optimal PCR performance specific for your assay.
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NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.
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