Reporter genes are attached to a regulatory sequence of a gene of interest. When expressed by the host organism, they generate measurable, easily identifiable characteristics that can be used as an indication of whether a gene has been taken up or expressed.
Recombinant human MET (amino acids 956-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MET is a proto-oncogene that encodes a transmembrane growth factor receptor.
Recombinant full-length human PKC iota was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Protein kinase C iota is a member of the protein kinase C family of serine-threonine kinases.
Recombinant human c-MER (amino acids 578-872) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. c-MER is a tyrosine kinase and a member of the MER/AXL/TYRO3 receptor kinase family.
Recombinant full-length human GSK3 beta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. GSK3 beta is a serine-threonine protein kinase originally identified as the kinase that phosphorylates and inhibits glycogen synthase.
Recombinant full-length human PKC zeta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC zeta is an atypical isoform of the PKC family.
Recombinant human CAMK2 gamma (C-terminal truncation) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. CAMK2 gamma is a member of the CAMKII family.
Recombinant human TRKA (amino acids 440-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TRKA is a member of the trk proto-oncogene family and encodes a 140kDa membrane-spanning protein tyrosine kinase.
Recombinant human FLT1 (amino acids 784-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. FLT1, also known as fms-like tyrosine kinase 1, is a member of the VEGFR family and is related to the oncogene ROS.
Recombinant human KDR (amino acids 789-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. KDR (or kinase insert domain receptor) is a growth factor receptor tyrosine kinase originally isolated from human endothelial cells.
PCR Optimization Kit is a portfolio of preformulated high-quality buffers, 25mM MgCl2 and GoTaq MDx Hot Start Polymerase. Perform a series of short experiments to quickly survey which combination will yield optimal PCR performance specific for your assay.
Promotes rapid lysis of cultured mammalian cells without the need to scrape adherent cells or freeze-thaw. Lytic reagent of choice when processing cells for quantitation of firefly and Renilla luciferase activities using the Dual-Luciferase(R) Assay.